From a legal standpoint, no.
If a "legal" appeal took place now, it could be decided by a different judge who could vacate Walker's decision with a different conclusion.
Keep in mind, Walker's decision, that a ban on SSM violated the Equal Protection clause of the Constitution, runs contrary to Baker v. Nelson, in that SCOTUS dismissed an appeal on exactly that grounds "for want of a substantial federal question".
Nothing that happened today puts a dent in the validity of Baker v. Nelson.