OK, Day 1 is in the books. My Princeton team ran 63 offensive plays, not including ones called back due to penalties. (I didn't count them in the PBP, I just added passing attempts and rushing carries.) Although I make use of two different OL position roles in my formations, in actuality both contain the same players in the top 5, and the same order of players for spots 6, 7, and 8.
My fatigue settings for OL is set for the default 85%. None of my players has a STA greater than 56. The lowest for any starter is 40. My #6 OL played 3% of the plays or 2 plays. My #7 OL played 1%, or 1 play. #8 did not get in the game. With 1:45 to go in the fourth quarter, all five starters were in the game showing at 90% fatigue or higher.
At 9:11 remaining in the fourth quarter, at the end of a 10 play drive, my #6 and #7 linemen finally got in the game, and the three remaining starters were still at 85%. We didn't have any more long drives in the game after that, which may have contributed to the results being the way they are.
On the defensive side of the ball, I see some drop off at the 4th DL, 4th DB, and 3rd LB slots, but that can be explained by using different defensive sets. All of the top depth chart players at those positions played well over 90%.
It's hard to tell whether or not the players' effectiveness was reduced by fatigue, but the amount of fatigue doesn't appear to be a factor in the game.
I think the >100% playing time is a result of counting penalties in the "plays participated in" but not n the "total number of plays"